目的 建立胶束电动毛细管色谱二极管阵列检测法(MEKC-DAD)同时测定不同产地及商品何首乌中二苯乙烯苷、大黄素、芦荟大黄素、大黄酸、大黄酚、大黄素甲醚、儿茶素含量的方法。 方法 选择胶束电动毛细管分离模式,用未涂渍标准熔融石英毛细管(75 μm×64.5 cm,有效长度56 cm)作为分离通道;背景电解质溶液:25 mmol·L-1硼砂-30 mmol·L-1 SDS-10%乙腈(pH 9.60);检测波长:254 nm;运行电压:25 kV;运行温度:24 ℃;压力进样:25 kPa,5 s。结果 何首乌中7种成分的浓度与峰面积的线性关系良好(r≥0.999 4);加样回收率为99.164%~101.504%。用此法测定何首乌中7种指标成分的含量,结果满意。 结论 该方法简单、准确,重现性好,为何首乌商品药材或饮片的质量评价和控制提供科学的参考依据。
Abstract
OBJECTIVE To establish a micellar electrokinetic chromatography with diode array detection (MEKC-DAD) method for simultaneous determination of seven components including stilbene glucoside, emodin, aloe-emodin, rhein, chrysophanol, physcion, and catechin in Polygoni Multiflori Radix. of different origins and commercial herbs. METHODS Based on the mode of MEKC, an uncoated fused silica capillary (75 μm × 64.5 cm, 56 cm of effective length ) was adopted, and 25 mmol·L-1 borax-30 mmol·L-1 SDS-10% acetonitrile (pH 9.60) was selected for the running buffer. The detection wavelength was set at 254 nm. The separation voltage was 25 kV and the column temperature was maintained at 24 ℃. The sample was injected at 5 kPa for 5 s. RESULTS The calibration curves of the seven components in Polygoni Multiflori Radix showed good linearity (r≥0.999 4). The average recoveries of the method were between 99.164%-101.504%. CONCLUSION The established method is reliable and accurate, and can be used for the quality control of Polygoni Multiflori Radix.
关键词
胶束电动毛细管电泳 /
何首乌 /
二苯乙烯苷 /
蒽醌 /
儿茶素 /
同时测定
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Key words
MEKC-DAD /
Polygoni Multiflori Radix /
stilbene glucoside /
anthraquinone /
catechin /
simultaneous determination
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中图分类号：
R284
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参考文献
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脚注
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基金
国家科技支撑计划项目资助项目(2011BAI13B04);江苏省中药炮制重点实验室开放式课资助项目(ZYPZ007);江苏高校优势学科建设工程资助项目资助项目(ysxk-2014)
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